L3771
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L3771
Sigma
Sodium dodecyl sulfate
for electrophoresis, ≥98.5% (GC)
| Synonym: | Dodecyl sodium sulfate, Dodecyl sulfate sodium salt, Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate |
| CAS Number: | 151-21-3 |
| Linear Formula: | CH3(CH2)11OSO3Na |
| Molecular Weight: | 288.38 |
| Beilstein Registry Number: | 3599286 |
| EC Number: | 205-788-1 |
| MDL number: | MFCD00036175 |
| PubChem Substance ID: | 24896351 |
Description
| Analysis Note | Tested for use in denatured polyacrylamide gel electrophoresis. |
| Application | Anionic detergent |
| Used to solubilize and denature proteins for denaturing-PAGE. Most proteins bind SDS in a ratio of 1.4 g SDS per gram of protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on their size. |
Properties
| grade | for electrophoresis |
| assay | ≥98.5% (GC) |
| mol wt | micellar mol wt 18,000 |
| aggregation number | 62 |
| turbidity | <2.5 NTU |
| CMC | 7-10 mM(20-25°C) |
| mp | 204-207 °C(lit.) |
| transition temp | cloud point >100 °C |
| anion traces | chloride (Cl-): ≤500 ppm |
| phosphate (PO4-): ≤10 ppm | |
| cation traces | heavy metals (as Pb): ≤10 ppm |
| abs. | A |
| A | |
| HLB | 40 |
Safety
| Personal Protective Equipment | dust mask type N95 (US), Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges |
| Hazard Codes | F,Xn |
| Risk Statements | 11-21/22-36/37/38 |
| Safety Statements | 26-36/37 |
| RIDADR | UN2926 4.1/PG 2 |
| WGK Germany | 2 |
| RTECS | WT1050000 |
| Flash Point(F) | 50 °F |
| Flash Point(C) | 10 °C |
References
| reference | Hames, B.D. and Rickwood, D. Gel Electrophoresis of Proteins: A Practical Approach 2nd ed., New York, NY , (1990), 8 |
| , Link, A.J., 2-D Proteome Analysis Protocols. Methods Mol. Biol. Totowa, NJ , (1999) 112 | |
| Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685, (1970) Abstract | |
| U.K. Laemmli, PAGE - a SDS-discontinuous system. Nature 227, 680, (1970) | |
| K. Harewood, J.S. Wolff, Anionic detergent for protein solubilization. Anal. Biochem. 55, 573, (1973) | |
| T.B. Nelsen, J.A. Reynolds, Measurement of molecular weight by gel electrophoresis. Meth. Enzymol. 48, 3, (1978) | |
| eds. L.A. Lewis, J.J. Oppelt CRC Handbook of Electrophoresis Boca Raton, Florida 1, (1980) | |
| R.A. Grohs, Analysis of drugs in the presence of serum albumin by LC with eluants containing surfactants. Anal. Chem. 63, 384, (1991) | |
| Merck | Merck 13,8710 |
| Beilstein | Beil. 1,IV,1847 |
| Aldrich MSDS 1, 847:C / Corp MSDS 1 (1), 1495:C / FT-IR 2 (1), 1532:B / FT-IR 1 (1), 904:C / IR-Spectra (3), 544:C / IR-Spectra (2), 481:G / NMR-Reference 2 (2), 805:A / RegBook 1 (1), 1079:E / Sax 6, 2490 / Sigma FT-IR 1 (2), 70:B / Sigma FT-IR 1 (2), 70:C / Structure Index 1, 167:C:8 |
